demultiplex_fasta.py: command not found

Have You tried accessing the binary with the full path?

Maybe Your $PATH variable doesn't contain the default paths? echo $PATH should give something like:

[root@server ~]# echo $PATH
/usr/local/sbin:/usr/local/bin:/sbin:/bin:/usr/sbin:/usr/bin:/root/bin

If it's empty, You may set the PATH variable with this command

export PATH="/usr/local/sbin:/usr/local/bin:/sbin:/bin:/usr/sbin:/usr/bin:/root/bin"

On Debian possible default full paths are:

/usr/lib/qiime/bin/demultiplex_fasta.py - from package: qiime

You may install the required package with command apt-get install {package}
Error in other languages:
demultiplex_fasta.py: bevel nie gevind nie
demultiplex_fasta.py: comandă negăsită
demultiplex_fasta.py: comando não encontrado
demultiplex_fasta.py: commande introuvable
demultiplex_fasta.py: command not found
demultiplex_fasta.py: käsku ei ole
demultiplex_fasta.py: không tìm thấy lệnh
demultiplex_fasta.py: komanda nerasta
demultiplex_fasta.py: Kommando nicht gefunden.
demultiplex_fasta.py: kommandot finns inte
demultiplex_fasta.py: komut yok
demultiplex_fasta.py: nie znaleziono polecenia
demultiplex_fasta.py: níor aimsíodh an t-ordú
demultiplex_fasta.py: no se encontró la orden
demultiplex_fasta.py: no s'ha trobat l'ordre
demultiplex_fasta.py: opdracht niet gevonden
demultiplex_fasta.py: parancs nem található
demultiplex_fasta.py: perintah tidak ditemukan
demultiplex_fasta.py: príkaz nenájdený
demultiplex_fasta.py: příkaz nenalezen
demultiplex_fasta.py: команда не найдена
demultiplex_fasta.py: командата не е открита
demultiplex_fasta.py: 命令找不到
demultiplex_fasta.py: コマンドが見つかりません